Brain-specific protein 14-3-2, an isoenzyme of enolase.
نویسنده
چکیده
x > c) .3 0.6 ...c) Vanaman, T. C., Sharief, F. & Watterson, D. M. (1977) in Walsh, M. P., Vallet, B., Autric, F. & Demaille, J. G. (1979) J . Biol. Calcium-Binding Proteins and Calcium Function (Wasserman, R. H., Corradino, R. A., Carafoli, E., Kretsinger, R. H., MacLennan, D. H. & Siegal, F. L., eds.), pp. 107-116, North-Holland, Wong, P. Y. K. & Cheung, W. Y. (1979) Biochem. Biophys. Res. Amsterdam Commun. 90,473-480 Yagi, K., Yazawa, M., Kakiuchi, S., Ohshima, M. & Uenishi, K. (1978) Biophys. Res. Commun. 65,975-981 Chem. 254, 12 136-12 144 Wang, J. H. & Desai, R. (1977) J. Biol. Chem. 252.4 175-4 I84
منابع مشابه
Brain-specific proteins: solid-phase immunobioluminescence assay for neuron-specific enolase in human plasma.
We raised specific antibodies in rabbits against pure alpha alpha- or gamma gamma-isoenzymes of enolase (EC 4.2.1.11) purified from human brain. After being specifically immunoabsorbed by the respective Sepharose-coupled enolase isoenzymes, the purified antibodies are desorbed by acidification and coated onto polystyrene tubes. Each sample containing the isoenzyme activity to be determined is i...
متن کاملUse of 14-3-3 and other brain-specific proteins in CSF in the diagnosis of variant Creutzfeldt-Jakob disease.
OBJECTIVES The detection of the protein 14-3-3 in the CSF has been shown to be a reliable and sensitive marker for sporadic Creutzfeldt-Jakob disease (CJD). Other brain-specific proteins such as neuron specific enolase (NSE), S-100b, and tau protein have also been reported to be increased in the CSF of patients with sporadic CJD. In 1996 a variant of CJD (vCJD) was described which is likely to ...
متن کاملMolecular evolution of enolase.
Enolase (EC 4.2.1.11) is an enzyme of the glycolytic pathway catalyzing the dehydratation reaction of 2-phosphoglycerate. In vertebrates the enzyme exists in three isoforms: alpha, beta and gamma. The amino-acid and nucleotide sequences deposited in the GenBank and SwissProt databases were subjected to analysis using the following bioinformatic programs: ClustalX, GeneDoc, MEGA2 and S.I.F.T. (s...
متن کاملBrain-derived proteins in the CSF, do they correlate with brain pathology in CJD?
BACKGROUND Brain derived proteins such as 14-3-3, neuron-specific enolase (NSE), S 100b, tau, phosphorylated tau and Abeta1-42 were found to be altered in the cerebrospinal fluid (CSF) in Creutzfeldt-Jakob disease (CJD) patients. The pathogenic mechanisms leading to these abnormalities are not known, but a relation to rapid neuronal damage is assumed. No systematic analysis on brain-derived pro...
متن کاملElectrophoretic assessment of aqueous and serum neurone-specific enolase in retinoblastoma and ocular malignant melanoma.
The isoenzyme pattern of enolase was examined in the aqueous humour and serum of patients with retinoblastoma (10 aqueous, 8 sera), malignant melanoma (4 aqueous, 25 sera), and normal subjects undergoing cataract surgery (25 aqueous, 30 sera). The assay we used allowed assessment of all three major isoenzymes, including the gamma gamma isoenzyme (neurone-specific enolase). No enolase was detect...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 8 5 شماره
صفحات -
تاریخ انتشار 1980